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| Registros recuperados: 11 | |
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| Mello,Sônia A.; Marese,Angélica C.M.; Brancalhão,Rose M.C.; Zanoni,Jacqueline N.; Natali,Maria Raquel M.. |
| During the ageing process the enteric nervous system undergoes morphofunctional changes, such as enteric neurodegeneration. Neuronal death can be attributed to increase radicals free, and ascorbic acid (AA), known antioxidant, could minimize damage cause by oxidative stress. The objective of this study is to analyse the behaviour of morphoquantative myenteric neurons in the duodenum of adult Wistar rats with aged 90 (C90), 345 (E345) and 428 (E428) days, as well as animals of the same age who received ascorbic acid supplementation for 120 days (EA345 and EA428). Whole-mount preparations of muscle layer from the duodenum of the animals were immunostained by the method myosin V. 80 microscopic fields were quantified (14.8 mm2/animal) and measured 100... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aging; Antioxidant; Enteric nervous system; Immunohistochemistry. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000100337 |
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| Santos,R.G.; Diniz,C.R.; Cordeiro,M.N.; De Lima,M.E.. |
| Tx1, a neurotoxin isolated from the venom of the South American spider Phoneutria nigriventer, produces tail elevation, behavioral excitation and spastic paralysis of the hind limbs after intracerebroventricular injection in mice. Since Tx1 contracts isolated guinea pig ileum, we have investigated the effect of this toxin on acetylcholine release, as well as its binding to myenteric plexus-longitudinal muscle membranes from the guinea pig ileum. [125I]-Tx1 binds specifically and with high affinity (Kd = 0.36 ± 0.02 nM) to a single, non-interacting (nH = 1.1), low capacity (Bmax 1.1 pmol/mg protein) binding site. In competition experiments using several compounds (including ion channel ligands), only PhTx2 and PhTx3 competed with [125I]-Tx1 for specific... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Phoneutria nigriventer; Spider toxins; Binding; Enteric nervous system; Calcium channels; Guinea pig ileum. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200019 |
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| Sant'Ana,Débora M.G.; Araújo,Eduardo J.A.; Ramos,Diego H.; Hermes-Uliana,Catchia; Natali,Maria Raquel M.. |
| The effects of severe protein malnutrition (4%) on myenteric neurons of Wistar rat duodenum, in relation to a standard 22%-protein diet for rodents, were assessed in this study. Segments of the duodenum from 10 rats from each nutritional group were submitted to the elaboration of whole mounts - 5 stained with Giemsa to determine the total population of myenteric neurons and the others stained by a histochemical method to detect nervous cells through the NADPH-diaphorase enzyme activity for studying the subpopulation of nitrergic neurons. The area of 100 neurons per animal, totalizing 2,000 neurons, were randomly measured by using the Image Pro-Plus®software. Malnourished rats presented 34.38% lower body weight and 10.60% duodenum length reduction when... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enteric nervous system; Malnutrition; Myenteric plexus; Nitrergic neurons. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000300022 |
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| Góis,Marcelo Biondaro; Hermes-Uliana,Catchia; Paltanin,Alessandra; Pontes,Willian; Araújo,Eduardo José De Almeida; Miranda Neto,Marcilio Hubner; Sant'Ana,Débora Mello Gonçales. |
| Enteric nervous plexuses have been the object of several studies, specially the myenteric plexus whose studies describe its organization, functions and alterations. On the other hand, the submucosal plexus has been less studied and still needs descriptive studies. To analyze morphologically and quantitatively submucosal neurons of the jejunum of 90-day-old healthy rats using different techniques for neuronal staining as a way to provide normality data to compare with future experimental studies. Whole mount preparations of the jejunum were submitted to Giemsa, NADH-diaphorase and NADPH-diaphorase techniques to stain the total neuronal population, more metabolically active subpopulation and subpopulation of nitrergic neurons, respectively. Neurons of the... |
| Tipo: Journal article |
Palavras-chave: Enteric nervous system; Meissner's plexus; Giemsa; NADH-diaphorase; NADPH-diaphorase. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000400050 |
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| Papazian-Cabanas,Rodrigo M.; Araújo,Eduardo J.A.; Silva,Aristeu V. da; Sant'Ana,Débora M.G.. |
| The effects of acute and chronic infection caused by Toxoplasma gondii on duodenal myenteric neurons were analyzed. Eighteen rats were assigned into four groups: Acute Control Group (ACG, n=4); Acute Experimental Group (AEG, n=4); Chronic Control Group (CCG, n=5); and Chronic Experimental Group (CEG, n=5). Rats from the AEG and CEG were inoculated orally with 105 genotype III (BTU-II strain) tachyzoites of T. gondii isolated from a dog with neurological signs. Acute groups were killed after 24 hours after the inoculation and the chronic groups after 30 days. Whole-mount from the duodenum were stained with Giemsa. The population density of myenteric neurons, as well the body cell, nuclear and cytoplasmic area were analyzed. Both acute and chronic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enteric nervous system; Infection; Parasite; Toxoplasmosis. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000300016 |
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| Moreira,Neide M; Sant'ana,Débora M. G; Araújo,Eduardo J. A; Toledo,Max J. O; Gomes,Mônica L; Araújo,Silvana M. de. |
| Define an experimental model by evaluating quantitative and morphometric changes in myenteric neurons of the colon of mice infected with Trypanosoma cruzi. Twenty-eight Swiss male mice were distributed into groups: control (CG, n=9) and inoculated with 100 (IG100, n=9) and 1000 (IG1000, n=10) blood trypomastigotes, Y strain-T. cruzi II. Parasitemia was evaluated from 3-25 days post inoculation (dpi) with parasites peak of 7.7 × 10(6) and 8.4 × 10(6) trypomastigotes/mL at 8th dpi (p>0.05) in IG100 and IG1000, respectively. Chronic phase of the infection was obtained with two doses of 100mg/Kg/weight and one dose of 250mg/Kg/weight of Benznidazole on 11, 16 and 18 dpi. Three animals from each group were euthanized at 18, 30 and 75 dpi. The colon was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chagas disease; Colon; Enteric nervous system; Giemsa; Trypanosoma cruzi. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652011000200014 |
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| Registros recuperados: 11 | |
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